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ATCC human colorectal cancer cell lines ht29
A. Percentage of Cleaved Caspase 3/7 positive <t>HT29</t> (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.
Human Colorectal Cancer Cell Lines Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colorectal cancer cell lines ht29/product/ATCC
Average 96 stars, based on 1 article reviews
human colorectal cancer cell lines ht29 - by Bioz Stars, 2026-02
96/100 stars
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99
ATCC human colon cancer cell line ht29
A. Percentage of Cleaved Caspase 3/7 positive <t>HT29</t> (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.
Human Colon Cancer Cell Line Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colon cancer cell line ht29/product/ATCC
Average 99 stars, based on 1 article reviews
human colon cancer cell line ht29 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

99
ATCC human colon cancer cell line ht29 cells
A. Percentage of Cleaved Caspase 3/7 positive <t>HT29</t> (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.
Human Colon Cancer Cell Line Ht29 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colon cancer cell line ht29 cells/product/ATCC
Average 99 stars, based on 1 article reviews
human colon cancer cell line ht29 cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

99
ATCC human colon cancer cell lines ht29
A. Percentage of Cleaved Caspase 3/7 positive <t>HT29</t> (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.
Human Colon Cancer Cell Lines Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colon cancer cell lines ht29/product/ATCC
Average 99 stars, based on 1 article reviews
human colon cancer cell lines ht29 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

99
ATCC human colorectal cancer cell line ht29
A. Percentage of Cleaved Caspase 3/7 positive <t>HT29</t> (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.
Human Colorectal Cancer Cell Line Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colorectal cancer cell line ht29/product/ATCC
Average 99 stars, based on 1 article reviews
human colorectal cancer cell line ht29 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

99
ATCC ht29 human colorectal cancer cell lines
The clinical significance of TIMP1 in CRC and in vitro study. A , B . Evaluation of silencing efficiency of siRNA in CRC cell lines; C . MTT and Cell colony forming assays are used to assess the influence of blocking TIMP1 expressions on proliferative abilities of HCT116 and <t>HT29</t> cells; D . The transwell assays revealed that silencing of TIMP1 inhibited the migration and invasion of CRC cells; E . The apoptosis observed after knocking down TIMP1 in CRC cells. All experiments and analyses were performed in triplicate to ensure accuracy and reliability. Numerical data are displayed as the mean ± standard deviation (SD). siRNA Small interfering RNA; Control: Blank control group; NC : Negative control group; ** means p value < 0.01; *** means p value < 0.001
Ht29 Human Colorectal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ht29 human colorectal cancer cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
ht29 human colorectal cancer cell lines - by Bioz Stars, 2026-02
99/100 stars
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A. Percentage of Cleaved Caspase 3/7 positive HT29 (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.

Journal: bioRxiv

Article Title: Molidustat Targets a Synthetic Lethal Vulnerability in APC-Mutant Colorectal Cancer through GSTP1 and PHD2 Co-Inhibition

doi: 10.64898/2026.01.31.702998

Figure Lengend Snippet: A. Percentage of Cleaved Caspase 3/7 positive HT29 (top), and RKO (bottom) cells. Cells were treated with Molidustat for 48 hours at indicated concentrations, 10uM Staurosporine was used as a positive control (100% cell death). Mean + SEM is assessed by unpaired two tailed Student’s t-test, **p<0.01, (ns) non-significant. B. Representative images of Cleaved Caspase-3/7 signal in DMSO, Molidustat (90 μM), and Staurosporine treated cells. Scale bar: 300 μm. C. Representative Western Blot of PHD2 levels in HT29 cells. D. Percentage confluency of HT29 cells post-transfection with the indicated guide RNAs. E. Cleaved Caspase-3/7 signal in HT29 cells post-transfection with the indicated crRNAs. Mean + SEM is assessed by two-way ANOVA, *p<0.05. N = 3 biologically independent experiments.

Article Snippet: Human colorectal cancer cell lines HT29 and RKO were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich, D6429) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, 16000044), 1% (v/v) penicillin-streptomycin (Gibco, 15140122), and 2 mM L-glutamine (Sigma-Aldrich, G7513).

Techniques: Positive Control, Two Tailed Test, Western Blot, Transfection

The clinical significance of TIMP1 in CRC and in vitro study. A , B . Evaluation of silencing efficiency of siRNA in CRC cell lines; C . MTT and Cell colony forming assays are used to assess the influence of blocking TIMP1 expressions on proliferative abilities of HCT116 and HT29 cells; D . The transwell assays revealed that silencing of TIMP1 inhibited the migration and invasion of CRC cells; E . The apoptosis observed after knocking down TIMP1 in CRC cells. All experiments and analyses were performed in triplicate to ensure accuracy and reliability. Numerical data are displayed as the mean ± standard deviation (SD). siRNA Small interfering RNA; Control: Blank control group; NC : Negative control group; ** means p value < 0.01; *** means p value < 0.001

Journal: Discover Oncology

Article Title: Bioinformatics mining and experimental validation of prognostic biomarkers in colorectal cancer

doi: 10.1007/s12672-025-03301-9

Figure Lengend Snippet: The clinical significance of TIMP1 in CRC and in vitro study. A , B . Evaluation of silencing efficiency of siRNA in CRC cell lines; C . MTT and Cell colony forming assays are used to assess the influence of blocking TIMP1 expressions on proliferative abilities of HCT116 and HT29 cells; D . The transwell assays revealed that silencing of TIMP1 inhibited the migration and invasion of CRC cells; E . The apoptosis observed after knocking down TIMP1 in CRC cells. All experiments and analyses were performed in triplicate to ensure accuracy and reliability. Numerical data are displayed as the mean ± standard deviation (SD). siRNA Small interfering RNA; Control: Blank control group; NC : Negative control group; ** means p value < 0.01; *** means p value < 0.001

Article Snippet: HCT116 and HT29 human colorectal cancer cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, USA) with 10% fetal bovine serum, and 1% penicillin and streptomycin.

Techniques: In Vitro, Blocking Assay, Migration, Standard Deviation, Small Interfering RNA, Control, Negative Control